The brief history of phenotype dissection of hematopoietic stem cells

by Alexey Bersenev on February 21, 2009 · 1 comment

in educational

I’m planning to give more educational information in different formats. I’d like to hear your opinion about what kind of info you want to see here and maybe suggest some resources.

Today is stem cell basics – historical overview of phenotype dissection of hematopoietic stem cells.

criteria of enrollment:
species: mice
organ: bone marrow
detection: by antibody to surface molecules (phenotype) by flow cytomentry

LSK = Lineage-negative (lin-), Sca-1+, c-Kit+ cells

Spangrude GJ, Heimfeld S, Weissman IL. Purification and characterization of mouse hematopoietic stem cells. Science. 1988;241: 58-62
Lab: Weissman IL

Ikuta K, Weissman IL. Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation. Proc Natl Acad Sci U S A. 1992;89: 1502-1506
Lab: Weissman IL

Osawa M, Hanada K, Hamada H, Nakauchi H. Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell. Science. 1996;273: 242-245
Lab: Nakauchi H

Adolfsson J, Borge OJ, Bryder D, et al. Upregulation of Flt3 expression within the bone marrow Lin(–)Sca1(+)c-kit(+) stem cell compartment is accompanied by loss of self-renewal capacity. Immunity. 2001;15: 659-669
Lab. Jacobsen SE

Christensen JL, Weissman IL. Flk-2 is a marker in hematopoietic stem cell differentiation: a simple method to isolate long-term stem cells. Proc Natl Acad Sci U S A. 2001;98: 14541-14546
Lab: Weissman IL

Yang L, Bryder D, Adolfsson J, Nygren J, Månsson R, Sigvardsson M, Jacobsen SE . Identification of Lin-Sca1+kit+CD34+Flt3- short-term hematopoietic stem cells capable of rapidly reconstituting and rescuing myeloablated transplant recipients. Blood. 2005 Apr 1;105(7):2717-23
Lab. Jacobsen SE

SLAM (CD150+/CD244-/CD48-)
Kiel MJ, Yilmaz OH, Iwashita T, Yilmaz OH, Terhorst C, Morrison SJ. SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell. 2005 Jul 1;121(7):1109-21
Lab: Morrison SJ

LSK/CD34-/Flk2-/SLAM (CD150+/CD41-/CD48-)
Wilson A, Laurenti E, Oser G, van der Wath RC, Blanco-Bose W, Jaworski M, Offner S, Dunant CF, Eshkind L, Bockamp E, Lió P, Macdonald HR, Trumpp A. Hematopoietic stem cells reversibly switch from dormancy to self-renewal during homeostasis and repair. Cell. 2008 Dec 12;135(6):1118-29
Lab: Trumpp A

Please feel free to correct or add more information!

{ 1 comment… read it below or add one }

Firas March 14, 2009 at 11:12 am

Hello Axel,

As I have just started my research in this field, I found this post very useful.
I encourage you to post more in this direction.

I am attending our journal clubs without having a hint where to start from. Everyone in my group is talking so professionally. So, thank you very much.



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